منابع مشابه
Preparation of pure lipid hydroperoxides.
Increasing evidence of lipid peroxidation in food deterioration and pathophysiology of diseases have revealed the need for a pure lipid hydroperoxide (LOOH) reference as an authentic standard for quantification and as a compound for biological studies in this field. Generally, LOOH is prepared from photo- or enzymatically oxidized lipids; however, separating LOOH from other oxidation products a...
متن کاملLipid hydroperoxides in plants.
Hydroperoxides are the primary oxygenated products of polyunsaturated fatty acids and were determined spectrophotometrically based on their reaction with an excess of Fe2+ at low pH in the presence of the dye Xylenol Orange. Triphenylphosphine-mediated hydroxide formation was used to authenticate the signal generated by the hydroperoxides. The method readily detected lipid peroxidation in a ran...
متن کاملOxidation of D-(-)-Ribose with H20 2 and Lipid Hydroperoxides
D-(-)-ribose was subjected to oxidation with equimolar amounts of H20 2/Fe2+ and 13-hydroperoxy-9-c/s-l l-fra/75-octadecadienoic acid/Fe2+. Smaller carbohydrates, carbonyl compounds, hydroxy acids and polyols were produced. Identification of carbonyl compounds was achieved by trapping with pentafluorobenzylhydroxylamine hydrochloride (PFBHA-HC1). All products were identified by GC/MS after deri...
متن کاملEvidence that lipid hydroperoxides inhibit plasma lecithin:cholesterol acyltransferase activity.
The oxidation of low density lipoproteins (LDL) has been implicated in the development of atherosclerosis. Recently, we found that polar lipids isolated from minimally oxidized LDL produced a dramatic inhibition of lecithin: cholesterol acyltransferase (LCAT) activity, suggesting that HDL-cholesterol transport may be impaired during early atherogenesis. In this study, we have identified molecul...
متن کاملEvidence for a concerted reaction between lipid hydroperoxides and polypeptides.
The events accompanying oxidative modification of low density lipoprotein (LDL) are multiple and complex, and the precise mechanisms remain to be determined. In the present studies, we examined a simple system in which we first prepared large amounts of lipid hydroperoxides (from linoleic acid or from phospholipids containing linoleic acid) by using soybean lipoxygenase (linoleate:oxygen oxidor...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 2008
ISSN: 0022-2275
DOI: 10.1194/jlr.d800034-jlr200